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O: Fachverband Oberflächenphysik
O 50: Poster Organic Molecules on Inorganic Substrates: Adsorption and Growth
O 50.4: Poster
Dienstag, 18. März 2025, 18:00–20:00, P2
Solvent-Free Fabrication of Supported Lipid Bilayers Incorporating Carbon Quantum Dots and Gramicidin A for Biosensing Applications — •Daniel A. Saavedra1, Benjamín Ruz1, Nicolás Moraga1, Nancy Gomez-Vierling1, Marcelo A. Cisternas2, Susana D. Rojas2, and Ulrich G. Volkmann1 — 1Instituto de Física, Pontificia Universidad Católica de Chile, Santiago, Chile — 2Escuela de Ingeniería Industrial, Univ. de Valparaíso, Chile
This study employed two phospholipids for the formation of supported lipid bilayers (SLBs): DPPG, with a net negative charge for enhanced adsorption to surfaces and biomolecules, and DPPC, which is neutral. SLBs were fabricated using a solvent-free, dry, ultra-high vacuum (UHV) approach [1], designed for protein insertion and nanoscale biosensor development for Forster energy transfer (FRET) detection mechanism.
Gramicidin A, a stable ion-channel protein, was incorporated into SLBs via physical vapor deposition monitored by high-resolution ellipsometry, followed by thermal treatment to enhance stability. Carbon quantum dots (CQDs), synthesized through hydrothermal carbonization of chitosan, were added via spin-coating. CQDs were selected for their photostability, low toxicity, and tunable fluorescence [2].
Acknowledgements: ANID Ph.D. Fellowships (DAS, NM, NGV), PUENTE-UC 2024-25. References: [1] Cisternas, M.A. et al., IJMS, 21, 6819 (2020). DOI: 10.3390/ijms21186819 [2] Wu, Q., et al., Nano Research, 16, 1835-1845 (2023). DOI: 10.1007/s12274-022-5189-2
Keywords: Solvent-Free Fabrication; Supported Lipid Bilayers (SLBs); Carbon Quantum Dots (CQDs); Gramicidin A Ion Channels; Biosensing Applications