Regensburg 2025 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 91: Focus Session Chemical Imaging for the Elucidation of Molecular Structure I (joint session O/BP)
O 91.1: Hauptvortrag
Donnerstag, 20. März 2025, 15:00–15:30, H24
Infrared Nanoscopy and Tomography of Intracellular Structures — Joachim Heberle1, Katerina Kanevche1, •Emmanuel Pfitzner1, David Burr2, Janina Drauschke2, Andreas Elsaesser2, and Jacek Kozuch1 — 1Freie Universität Berlin, Department of Physics, Experimental Molecular Biophysics, — 2Experimental Biophysics and Space Sciences, Arnimallee 14, 14195, Berlin, Germany
Although techniques such as fluorescence-based super-resolution imaging or confocal microscopy simultaneously gather morphological and chemical data, these techniques often rely on localized and chemically specific markers. To eliminate this flaw, we have developed a method of examining cellular cross-sections using the imaging power of scattering-type scanning near-field optical microscopy (sSNOM) and Fourier-transform infrared spectroscopy at a spatial resolution far beyond the diffraction limit (nanoFTIR). Herewith, nanoscale surface and volumetric chemical imaging are performed using the intrinsic contrast generated by the characteristic absorption of mid-infrared radiation by the covalent bonds. We employ infrared nanoscopy to study the subcellular structures of eukaryotic (C. reinhardtii) and prokaryotic (E. coli) species, revealing chemically distinct regions within each cell. Serial 100 nm-thick cellular cross-sections were compiled into a tomogram, yielding a three-dimensional infrared image of subcellular structure distribution at 20 nm spatial resolution. The presented methodology can image biological samples with less interference due to the low energy of infrared radiation and the absence of labeling.
Keywords: infrared; imaging; subcellular; optical nearfield; sSNOM