Osnabrück 2002 – scientific programme

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MO: Molekülphysik

MO 7: Poster I (Experimentelle Techniken, Spektroskopie, Cluster)

MO 7.6: Poster

Wednesday, March 6, 2002, 11:00–13:00, Schloss

Combining Atomic Force Microscopy and Optical Single Molecule Microscopy — •O. Panzer, U.C. Fischer, A. Naber, and H. Fuchs — Physikalisches Inst. der Universität Münster

For several years a number of techniques made it possible to detect the fluorescence of single molecules. The characteristics of many fluorophores were explored since, they were utilized as probes of their nano-environment and phenomena specific to single molecules were observed for the first time.

Very recently a variety of experiments have been proposed to manipulate the optical properties of single fluorophores in a controlled manner, mostly by energy transfer to a functionalised probe.

To enhance the spatial localisation of single molecule fluorescence microscopy we want to make use of short ranged fluorescence-quenching in the near-field of the metalized tip of an atomic force microscope (afm). Therefore we combined a scanning confocal fluorescence microscope with a tuning fork type afm. Here we present first results concerning the apparatus and samples.