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SYBM: Physik biologischer Materie

SYBM 401: Physics of biological matter: Poster

SYBM 401.12: Poster

Dienstag, 12. März 2002, 18:00–19:30, H37

Mechanics, Stress Fiber Formation and Force Generation of a single GFP-actin Fibroblast — •Alexandre Micoulet¹, Joachim Spatz¹, and Albrecht Ott² — ¹University of Heidelberg, Institute of Physical Chemistry, Biophysical chemistry, Im Neuenheimer Feld 253, D-69120 Heidelberg — ²University of Bayreuth, Chair of Experimental Physics I, D-95440 Bayreuth

Differentiation and genetic expression of cells as well as development of first embryonic states are constantly regulated processes where biochemical and mechanical interactions between a cell and its environment play a major role. We want to stimulate a cell by defined biochemical receptors and external mechanical stress. The chemical-physical properties of synthetic macromolecules and peptides have been used to modify surfaces on the molecular level. These surfaces were used to manipulate the adhesion and motility properties of GFP-actin fibroblast cells. The interaction of cells with such surfaces were studied by applying a uniaxial force to an individual cell. Conditions of constant stress or constant strain allowed the determination of the dynamic reaction of a cell to an external, mechanical stimulation. By using GFP-actin fibroblast cells, the dynamic development of actin filaments were monitored in-situ during defined external stimulation.