Dresden 2003 – wissenschaftliches Programm

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CPP: Chemische Physik und Polymerphysik

CPP 10: Experimente mit Einzelmolekülen

CPP 10.6: Vortrag

Donnerstag, 27. März 2003, 11:15–11:30, ZEU/160

Single molecule level detection of hydrophobicity change during GroEL activity by Atomic Force Microscopy — •Giovanni Dietler¹, Francesco Valle¹, James A. DeRose¹, Gerit Kulik¹, Martin Kawe², and Andreas Plückthun² — ¹Inst. of Condensed Matter Physics, University of Lausanne, CH-1015 Lausanne, Switzerland — ²Biochemisches Institut der Universität Zürich, CH-8057 Zürich, Switzerland

GroEL is a protein complex with a toroidal shape and a central cavity. GroEL, a chaperonin molecule, folds misfolded or newly synthetized proteins. The activity cycle of GroEL implies a change of the central cavity from hydrophobic to hydrophilic character. By means of the Atomic Force Microscopy it was possible to show at the single molecule level that the central cavity changes from hydrophobic to hydrophilic during the ATP cycle. This measurement was carried out by exploiting the attractive or repulsive interaction between the GroEL cavity and the AFM tip.