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Berlin 2005 – scientific programme

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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.82: Poster

Saturday, March 5, 2005, 16:45–18:45, Poster TU D

In-plane and out-of-plane fluctuation of synthetic glycolipid lamellae under controlled osmotic pressure and temperature — •Emanuel Schneck1, Florian Rehfeldt1, Bruno Demé2, and Motomu Tanaka11Technische Universität München — 2Institut Laue-Langevin

The in-plane and out of plane cooperativity in artificial models of cell glycocalix was studied using the D16 membrane diffractometer coupled with a climate chamber. Rocking curves of oriented multilamellar stacks using the 2D detector (reciprocal space maps, q vs. qx) allow for the analysis of the scattering along different orientations referring to in-plane and out-of-plane contributions independently. In-plane membrane fluctuations produce diffuse scattering along q, while fluctuations of the periodicity affects the sharpness of the Bragg peaks along qz (specular reflectivity). The analysis of the measured lamellar periodicities yields quantitative force-distance relationships, which clearly reveal the competitive interplays of repulsive hydration forces and attractive NodqzipperNodq forces depending on the conformation of carbohydrate head groups.

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