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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.82: Poster

Samstag, 5. März 2005, 16:45–18:45, Poster TU D

In-plane and out-of-plane fluctuation of synthetic glycolipid lamellae under controlled osmotic pressure and temperature — •Emanuel Schneck¹, Florian Rehfeldt¹, Bruno Demé², and Motomu Tanaka¹ — ¹Technische Universität München — ²Institut Laue-Langevin

The in-plane and out of plane cooperativity in artificial models of cell glycocalix was studied using the D16 membrane diffractometer coupled with a climate chamber. Rocking curves of oriented multilamellar stacks using the 2D detector (reciprocal space maps, q_∥ vs. q_x) allow for the analysis of the scattering along different orientations referring to in-plane and out-of-plane contributions independently. In-plane membrane fluctuations produce diffuse scattering along q_∥, while fluctuations of the periodicity affects the sharpness of the Bragg peaks along q_z (specular reflectivity). The analysis of the measured lamellar periodicities yields quantitative force-distance relationships, which clearly reveal the competitive interplays of repulsive hydration forces and attractive No−dqzipperNo−dq forces depending on the conformation of carbohydrate head groups.