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BP: Fachverband Biologische Physik

BP 26: Poster Session II

BP 26.38: Poster

Donnerstag, 29. März 2007, 17:00–19:30, Poster B

Preparation of horizontal black lipid bilayers incorporated in a microfluidics system for microscopy and industrial parallelization — •Tivadar Mach¹, Claus Fütterer¹, Jürgen Fritz¹, Niels Fertig², Catalin Chimerel¹, and Mathias Winterhalter¹ — ¹International University Bremen, Bremen, Germany — ²Nanion GmbH, München, Germany

A planar black lipid membrane, widely used for electrophysiological studies, is reconstituted on a micron-size glass aperture inside a microfluidic chip, forming a GΩ seal using giant liposome adsorption and rupture. This novel system offers very low noise recordings (under 1 pA RMS at 10 kHz, essentially equal to the open headstage noise in our system), complete control of the measurement environment, access to the bilayer from both sides, the use of μl analyte volumes, and a great potential for automation and parallelization. Minimizing the microfluidics thickness on the lower side of the BLM enables us to approach the bilayer with a 100x objective making concurrent electrophysiological and fluorescence microscopy studies possible.