Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster I
BP 7.35: Poster
Montag, 23. März 2009, 17:45–20:00, P3
Three-bead assay for single molecule ncd study — •Christoph Pieper1, Li Tao2, Kerstin von Roden1, Stefan Lakämper1, Jonathan Scholey2, and Christoph Schmidt1 — 13. Physikalisches Institut, Georg-August-Universität, 37077 Göttingen — 2Department of Molecular and Cell Biology, University of California at Davis, Davis, CA 95616
The ncd protein is a dimeric minus-end directed motor of the kinesin family. Previous experiments using optical tweezers and a three-bead, suspended-micotubule assay showed transient microtubule-binding events with a working stroke of about 9 nm at the end of the binding events. Binding events could be detected by the reduction in noise of the suspended microtubule. We have now developed an advanced assay, using a new FPGA board for the rapid online detection of these binding events. The FPGA further controls acousto-optical deflectors for beam steering and trap feedback. With this system feedback can activated on ncd binding to study the behavior of a single ncd motor under a defined load.