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BP: Fachverband Biologische Physik

BP 7: Poster I

BP 7.35: Poster

Montag, 23. März 2009, 17:45–20:00, P3

Three-bead assay for single molecule ncd study — •Christoph Pieper¹, Li Tao², Kerstin von Roden¹, Stefan Lakämper¹, Jonathan Scholey², and Christoph Schmidt¹ — ¹3. Physikalisches Institut, Georg-August-Universität, 37077 Göttingen — ²Department of Molecular and Cell Biology, University of California at Davis, Davis, CA 95616

The ncd protein is a dimeric minus-end directed motor of the kinesin family. Previous experiments using optical tweezers and a three-bead, suspended-micotubule assay showed transient microtubule-binding events with a working stroke of about 9 nm at the end of the binding events. Binding events could be detected by the reduction in noise of the suspended microtubule. We have now developed an advanced assay, using a new FPGA board for the rapid online detection of these binding events. The FPGA further controls acousto-optical deflectors for beam steering and trap feedback. With this system feedback can activated on ncd binding to study the behavior of a single ncd motor under a defined load.