Hannover 2010 – wissenschaftliches Programm

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Q: Fachverband Quantenoptik und Photonik

Q 55: Poster II

Q 55.82: Poster

Donnerstag, 11. März 2010, 16:00–19:00, Lichthof

Realization of a scanning microscope for nonlinear microscopy — •Sebastian Beer^1,2, Petra Groß¹, Carsten Cleff¹, Lisa Kleinschmidt¹, and Carsten Fallnich¹ — ¹Institut für Angewandte Physik, Westfälische Wilhelms-Universität, Corrensstr. 2, 48149 Münster — ²Fachhochschule Gießen-Friedberg, Wiesenstr. 14, 35390 Gießen

During the last decade, research in biology and the life sciences has inspired and led to major achievements in nonlinear microscopy. This includes the improvement of resolution beyond the diffraction limit as well as advances in and application of different nonlinear microscopy methods like two-photon excited fluorescence, harmonic generation or coherent anti-Stokes Raman scattering (CARS). Typically, these methods require scanning of the focus across the sample.

We have developed a multimodal scanning microscope, where not the focus, but the sample is scanned in order to disturb the focus as little as possible. The easy access to all building blocks and the resulting flexibility made an experimental setup more attractive than a commercial, but expensive microscope. Using a piezo-driven three axis stage, the specimen can be scanned by 150 µm in each direction. We present practical considerations and technical details which one has to keep in mind when constructing a high performance scanning microscope. We also show first results obtained by two-photon excited fluorescence-, second harmonic generation-, and CARS-microscopy, which demonstrate that our microscope is highly suitable for multimodal nonlinear microscopy.