Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 33: Posters: Neurobiophysics

BP 33.3: Poster

Donnerstag, 25. März 2010, 17:15–20:00, Poster B1

Hidden Markov Models reveal distinct mobilities of synaptic vesicles — •Jan-Philipp Spies¹, Christoph Erlenkämper¹, Mathias Pasche², Detlef Hof², Karsten Kruse¹, Jens Rettig², and Ute Becherer² — ¹Theoretische Biologische Physik, Universität des Saarlandes, 66041 Saarbrücken — ²Physiologisches Institut, Universität des Saarlandes, 66421 Homburg

In neurons, release of neurotransmitter occurs through fusion of synaptic vesicles with the plasma membrane. Electrophysiological methods, e.g. membrane capacitance measurements, provide indirect information about distinct vesicle states ("docking" and "priming"). At present, the molecular origin of these states is unknown. To characterize them on the level of individual vesicles, we investigate their mobility using total internal reflection fluorescence (TIRF) microscopy. Employing Hidden Markov Models, we identify several states of different mobilities and propose possible underlying molecular mechanisms.