Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 33: Posters: Neurobiophysics
BP 33.3: Poster
Donnerstag, 25. März 2010, 17:15–20:00, Poster B1
Hidden Markov Models reveal distinct mobilities of synaptic vesicles — •Jan-Philipp Spies1, Christoph Erlenkämper1, Mathias Pasche2, Detlef Hof2, Karsten Kruse1, Jens Rettig2, and Ute Becherer2 — 1Theoretische Biologische Physik, Universität des Saarlandes, 66041 Saarbrücken — 2Physiologisches Institut, Universität des Saarlandes, 66421 Homburg
In neurons, release of neurotransmitter occurs through fusion of synaptic vesicles with the plasma membrane. Electrophysiological methods, e.g. membrane capacitance measurements, provide indirect information about distinct vesicle states ("docking" and "priming"). At present, the molecular origin of these states is unknown. To characterize them on the level of individual vesicles, we investigate their mobility using total internal reflection fluorescence (TIRF) microscopy. Employing Hidden Markov Models, we identify several states of different mobilities and propose possible underlying molecular mechanisms.