Regensburg 2013 – wissenschaftliches Programm

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O: Fachverband Oberflächenphysik

O 36: Poster Session II (Organic films and electronics, photoorganics; Nanostructures; Plasmonics and nanooptics, Surface chemical reactions and heterogeneous catalysis, Surface dynamics )

O 36.58: Poster

Dienstag, 12. März 2013, 18:15–21:45, Poster B2

New approach to the fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures — •M Nuruzzaman Khan¹, Vinalia Tjong², Ashutosh Chilkoti², and Michael Zharnikov¹ — ¹Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg, Germany — ²Department of Biomedical Engineering, Duke University, N C 27708-0300, USA

Immobilization of single stranded DNA (ssDNA) on solid support is an important issue for binding and detection of the complementary DNA target as well as for the recognition of DNA binding proteins. Here we present a universal two-step approach to fabricate mixed monomolecular films comprising thiolated ssDNA and oligo(ethylene glycole) substituted alkanethiolates (OEG-AT) which exhibit biocompatible properties providing a suitable matrix for the ssDNA . At first, the primary OEG-AT monolayer was irradiated with electrons to create defects in a controlled fashion. In the second step, a defect-promoted exchange between the molecules in the monolayer and ssDNA species in solution occurred, with the extent depending on the irradiation dose. The approach was combined with E-beam lithography, which allowed us to fabricate versatile ssDNA patterns of any required shape imbedded in the protein-repelling matrix. Further, applying surface initiated enzymatic polymerization, we succeeded to amplify the above ssDNA/OEG-AT patterns in the z-direction in a controlled fashion. Both ssDNA pattern and related 3D nanostructures can be widely used as versatile nanofabrication platform in such important fields as bio-engineering, bio-technology, and sensor fabrication.