Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 16: Posters: Cell adhesion, mechanics and migration

BP 16.11: Poster

Montag, 16. März 2015, 17:30–19:30, Poster A

Topography and elasticity measurements on squamous carcinoma cells by AFM — •Tanja Schreyer¹, Susanne Steeger¹, Stefan Hansen², Jörg Schipper², and Mathias Getzlaff¹ — ¹Heinrich-Heine-Universität Düsseldorf, Deutschland — ²Univ.-HNO-Klinik Düsseldorf, Deutschland

In this contribution we report on measurements of the mechanoelastic properties of squamous carcinoma cells. This study of single cancer cells in culture medium is carried out by Atomic Force Microscopy. In order to determine the elasticity of the cancer cells by calculating the Youngs modulus in the Hertz model we take force distance curves. We analyse and compare the different stiffnesses dependent on a position within one single cell. Accordingly we are able to avoid interfering effects of the substrate. This strategy is applied to the different densely populated areas of a cell culture. In addition we are interested in the various topography of the cells within a cell culture. In this way we are able to characterise the different cell types of the culture in order to relate their stiffness and their visual appearence.