Berlin 2015 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 39: Cell adhesion, mechanics and migration I (Joint session BP, CPP)

CPP 39.11: Vortrag

Mittwoch, 18. März 2015, 12:45–13:00, H 1058

Properties of Single Squamous Cell Carcinoma Cells — •Susanne Steeger¹, Tanja Schreyer¹, Stefan Hansen², Jörg Schipper², and Mathias Getzlaff¹ — ¹Heinrich-Heine-Universität Düsseldorf, Deutschland — ²Univ.-HNO-Klinik Düsseldorf, Deutschland

In this contribution we report on measurements of the mechanoelastic properties of ENT squamous cell carcinoma cells. The study of these single cancer cells in culture medium is carried out by Atomic Force Microscopy. Our main interest is the determination oft the Youngs Modulus calculated by the Hertzian Model. We identify the elasticity of cancer cells in order to compare it with that of similar benign cells. Because Live Cell Imaging is a challenging task we first focus on testing different cantilevers and various strategies to treat the cells carefully. In order to determine the individual properties of the cancer cells we additionally analyse their cytoskeleton (actin and tubulin) by using a confocal fluorescence microscope. Cancer cells are known for their modified cytosekelton which is reflected in the different elasticities of both cancer and comparable benign cells.