Berlin 2015 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 39: Cell adhesion, mechanics and migration I (Joint session BP, CPP)
CPP 39.11: Talk
Wednesday, March 18, 2015, 12:45–13:00, H 1058
Properties of Single Squamous Cell Carcinoma Cells — •Susanne Steeger1, Tanja Schreyer1, Stefan Hansen2, Jörg Schipper2, and Mathias Getzlaff1 — 1Heinrich-Heine-Universität Düsseldorf, Deutschland — 2Univ.-HNO-Klinik Düsseldorf, Deutschland
In this contribution we report on measurements of the mechanoelastic properties of ENT squamous cell carcinoma cells. The study of these single cancer cells in culture medium is carried out by Atomic Force Microscopy. Our main interest is the determination oft the Youngs Modulus calculated by the Hertzian Model. We identify the elasticity of cancer cells in order to compare it with that of similar benign cells. Because Live Cell Imaging is a challenging task we first focus on testing different cantilevers and various strategies to treat the cells carefully. In order to determine the individual properties of the cancer cells we additionally analyse their cytoskeleton (actin and tubulin) by using a confocal fluorescence microscope. Cancer cells are known for their modified cytosekelton which is reflected in the different elasticities of both cancer and comparable benign cells.