Regensburg 2016 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
CPP: Fachverband Chemische Physik und Polymerphysik
CPP 45: Poster: Two Dimensional Functional Materials
CPP 45.3: Poster
Mittwoch, 9. März 2016, 18:15–21:00, Poster B2
Assembly of proteins and oriented purple membrane on functionalized carbon nanomembranes — Natalie Frese1, •Paul Penner1, Matthias Schürmann1, Daniel Rhinow2, Mark Schnietz1, Polina Angelova1, Andrey Turchanin3, Christian Kaltschmidt1, Robert Tampé4, Norbert Hampp5, and Armin Gölzhäuser1 — 1University of Bielefeld — 2Max Planck Institute of Biophysics, Frankfurt — 3Friedrich Schiller Universtity Jena — 4Goethe University, Frankfurt — 5Philipps University, Marburg
This presentation is about hybrid structures comprising carbon nanomembrane (CNM) as a functional substrate and oriented assembled purple membranes (PMs). CNMs are monomolecular cross-linked layers of aromatic amphiphilic molecules with lateral dimensions of several square centimeters and a thickness of about 1 nm. PM from Halobacterium salinarum is a membrane consisting of bacteriorhodopsin (BR), which is a light-driven proton pump, and lipids. CNM has already been successfully tested as a substrate for electron cryo-microscopy of PM. To realize the oriented assembly of PM patches on CNM, we used a PM mutant, which has histidine (HIS) tags selectively on one side of the membrane and a nitroliotriacetic acid (NTA) terminated NBPT-CNM. The functionalized CNM has also been tested with different HIS-tagged proteins.