Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Posters - Bioimaging and Spectroscopy
BP 8.3: Poster
Monday, March 20, 2017, 17:30–19:30, P3
Staining of squamous cell carcinoma cells and dysplastic oral keratinocytes — •Jan Lietz1, Maja Strugacevac1, Ayse Almaci1, Julia Kristin2, Marcel Glaas2, Jörg Schipper2, and Mathias Getzlaff1 — 1Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf — 2Hals-Nasen-Ohrenklinik, Universitätsklinikum Düsseldorf
The goal of this research is to investigate the differences between oral carcinoma cells (UD-SCC-1) and dysplastic oral keratinocytes (DOK). In order to obtain more information about cell properties confocal laser scanning microscopy is used.
The cell membrane as well as the cytoskeleton, mitochondria and nucleus of both, squamous cell carcinoma cells and oral keratinocytes, were stained with CellMask green, SiR-tubulin and SiR-actin, MitoTracker orange and Hoechst 33342, respectively.
This contribution focuses on combining two, three and four different fluorescent dyes and optimizing the staining process accordingly. In addition, we acquired z-stacks and present reconstructed 3D images for each of the two cell lines.