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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.3: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Staining of squamous cell carcinoma cells and dysplastic oral keratinocytes — •Jan Lietz¹, Maja Strugacevac¹, Ayse Almaci¹, Julia Kristin², Marcel Glaas², Jörg Schipper², and Mathias Getzlaff¹ — ¹Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf — ²Hals-Nasen-Ohrenklinik, Universitätsklinikum Düsseldorf

The goal of this research is to investigate the differences between oral carcinoma cells (UD-SCC-1) and dysplastic oral keratinocytes (DOK). In order to obtain more information about cell properties confocal laser scanning microscopy is used.

The cell membrane as well as the cytoskeleton, mitochondria and nucleus of both, squamous cell carcinoma cells and oral keratinocytes, were stained with CellMask green, SiR-tubulin and SiR-actin, MitoTracker orange and Hoechst 33342, respectively.

This contribution focuses on combining two, three and four different fluorescent dyes and optimizing the staining process accordingly. In addition, we acquired z-stacks and present reconstructed 3D images for each of the two cell lines.