Dresden 2017 – wissenschaftliches Programm
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DS: Fachverband Dünne Schichten
DS 44: Postersession II
DS 44.44: Poster
Donnerstag, 23. März 2017, 17:00–19:00, P1C
X-Ray Reflectivity Investigation of Structure and Kinetics of Photoswitchable Lipid Monolayers — •Malte Jacobsen1, Kuntal Chatterjee1, Björn Haushahn1, Chen Shen1,2, Sven Festersen1, Jonas Warias1, Benjamin Runge1, Franziska Reise3, Thisbe Lindhorst3, Beate Maria Klösgen2, Olaf Magnussen1,4, and Bridget Murphy1,4 — 1Institute for Experimental and Applied Physics, CAU Kiel, Germany — 2SDU Odense, Denmark — 3Institute of Organic Chemistry, CAU Kiel, Germany — 4Ruprecht Haensel Laboratory, CAU Kiel, Germany
The mechanical and dynamic properties of phospholipid membranes are important for biological functions such as switching of embedded proteins. To investigate these biologically relevant properties we study model systems in which amphiphilic photoswitchable glycoconjugates have been integrated into biomimetic phospholipid membranes. The structure and kinetics of the switching process in a lipid Langmuir film are measured using Langmuir isotherms and in situ X-ray reflectivity at LISA diffractometer P08, PETRA III. We have modified glycolipids to contain an azobenzene photoswitch between the chain and head group and successfully embedded them in a monolayer of DPPC. We can reversibly change the azobenzene-glycolipid orientation between trans- and cis-conformation by illumination with UV and blue light. Strong changes in membrane conformation upon switching have been observed, and a critical point has been discovered above which the membrane expands rather than compresses upon switching. This work is funded by SFB 677 and BMBF 05K13FK2.