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BP: Fachverband Biologische Physik

BP 33: Protein Structure and Dynamics

BP 33.1: Vortrag

Donnerstag, 19. März 2020, 09:30–09:45, ZEU 250

Protein Short-Time Diffusion in a Naturally Crowded Environment — Marco Grimaldo¹, Hender Lopez^1,2,3, •Christian Beck^1,2, Olga Matsarskaia¹, Felix Roosen-Runge⁵, Martine Moulin¹, Juliette Devos¹, Valerie Laux¹, Michael Hartlein¹, Stefano Da Vela², Ralf Schweins¹, Alessandro Mariani⁴, Fajun Zhang², Jean-Louis Barrat³, Martin Oettel², V. Trevor Forsyth^1,6, Tilo Seydel¹, and Frank Schreiber² — ¹Institut Laue-Langevin, Grenoble, France — ²University of Tübingen, Germany — ³LiPhy, Saint Martin d’Hères, France — ⁴European Synchrotron Radiation Facility, Grenoble, France — ⁵Malmö University, Malmö, Sweden — ⁶Keele University, Staffordshire, UK

We employ neutron backscattering spectroscopy to measure the short-time self-diffusion of tracer proteins in a deuterated cell-like environment (cell lysate) with explicit control over crowding conditions. We successfully link coarse-grained Stokesian dynamics simulations with experimental results on these complex, flexible molecules providing a consistent understanding by colloid theories. In the case of immunoglobulin, both experiments and simulations show that tracers in polydisperse solutions close to the effective particle radius R_eff=⟨ R_i³⟩^1/3 diffuse approximately as if the suspension was monodisperse [1]. The simulations predict size-dependent deviations from this scaling which was tested by measuring different proteins in lysate with an increased energy transfer range, also allowing to investigate the influence of the lysate on the internal dynamics in more detail. [1] M. Grimaldo et al.; J. Phys. Chem. Lett. 10 (2019) 1709