Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.5: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Quantification of molecule-spanning protein dynamics with fluorescence correlation spectroscopy — •Veronika Frank1, Jean-Benoît Claude2, Jérôme Wenger2, and Thorsten Hugel1,3 — 1Institute of Physical Chemistry, University of Freiburg, Germany — 2Fresnel Institute, CNRS, Aix Marseille University, France — 3Signaling research centers BIOSS and CIBSS, University of Freiburg, Germany
Protein conformational kinetics and their regulation occur on many time and length scales. Fluorescence methods have mainly focused on the hundreds of microseconds to minutes time scale and NMR on the picosecond time scale. Here we explore the several nanoseconds to microseconds time scale for the multi-domain molecular chaperone heat shock protein Hsp90, a homodimer with a molecular weight of 90 kDa per monomer. Hsp90 is a therapeutic target for cancer therapy, but its dynamics and its dynamic interactions with other proteins are not yet fully understood.
Here we use fluorescence correlation spectroscopy and zero-mode waveguide nanoapertures to measure and understand fast molecule-spanning dynamics and how they are affected by interactors.