SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Tissue Mechanics II

BP 15.5: Vortrag

Mittwoch, 29. März 2023, 11:45–12:00, BAR Schö

Capturing the mechanosensitivity of cell proliferation in models of epithelium — •Maxime Hubert¹, Kevin Höllring¹, Lovro Nuić², Luka Rogić², Sara Kaliman¹, Simone Gehrer¹, Florian Rehfeldt^3,4, and Ana-Sunčana Smith^1,2 — ¹FAU Erlangen-Nürnberg, Erlangen, Germany — ²Ruđer Bošković Institute, Zagreb, Croatia — ³University of Göttingen, Göttingen, Germany — ⁴University of Bayreuth, Bayreuth, Germany

The proliferation of epithelial cells, the process of cell growth and cell division, is affected by the mechanical properties of the surrounding environment. While an extensive literature covers single cell mechanoresponse, information about tissue-wide mechanoresponse are scarce. It is only known that high cell density restricts proliferation and eventually leads to homeostasis. In this presentation, we aim at completing the existing literature by addressing the role of both cell density and extracellular stiffness in the proliferation of cells in epithalial monolayer. Using MDCK-II epithelial tissues stained with EdU we are able to measure the fraction of dividing cells at a given cell density and quantify mechanoresponse. We build a cell-level theory of proliferation based on a two-population description which is compared successfully to the experiments and implemented into simulations. Using experiments and simulations, we also address the role of proliferation in the large scale growth of tissues. A tissue-scale theory of epithelial growth based on the cell-level findings is finally presented. This work provides a first step towards a complete description of proliferation at the tissue scale and its influence on its compartmentalization.