Berlin 2024 – scientific programme
Parts | Days | Selection | Search | Updates | Downloads | Help
BP: Fachverband Biologische Physik
BP 21: Poster IIIb
BP 21.22: Poster
Wednesday, March 20, 2024, 11:00–14:30, Poster C
A NAP-XPS-study on X-ray radiation damage: Chemical changes to Gene-V Protein — •Dorothea C. Hallier1, 2, 3, Jörg Radnik2, Paul M. Dietrich4, Harald Seitz1, 3, and Marc Benjamin Hahn2 — 1Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — 2Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — 3Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — 4SPECS Surface Nano Analysis GmbH, Berlin, Germany
Single-stranded DNA-binding proteins such as Gene-V Protein (G5P/GVP) are involved in maintaining the DNA metabolism in cells. This is essential for cell viability, especially after exposure to ionizing radiation, i.e. after radiation therapy in cancer treatment. X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to G5P itself. Direct and indirect damage was detected through combined vacuum XPS and near-ambient pressure (NAP) XPS measurements under water atmosphere. A strong increase of protein damage was observed in water as compared to vacuum.
Keywords: radiation damage; X-ray; ssDNA-binding protein; XPS; NAP XPS