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Berlin 2024 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Poster Session Ia

BP 8.2: Poster

Monday, March 18, 2024, 18:00–20:30, Poster C

Imaging F-Actin arrangement via homo-FRET using 2D polarization fluorescence microscopyLukas Spantzel1,2, Chen Sun3, Lena Jesse1,2, Yunhao Mei1,4, Yutong Wang1,4, Shangjun Cheng1,2,4, Mohammad Soltaninezhad1,4, Michael Börsch1,2, Rainer Heintzmann1,4, Ivan G. Scheblykin3, Adrian T. Press1,2, and •Daniela Täuber1,41Friedrich Schiller University, Jena — 2University Hospital Jena — 3Lund University, Sweden — 4Leibniz Institute of Photonic Technology, Jena, Germany

Polymicrobial infection affects the organization of F-Actin in the cytoskeleton and the cortex causing cell death. We use 2-dimensional polarization fluorescence imaging (2DPOLIM) to visualize the aggregation of phalloidin-dye labeled F-Actin via Förster Resonance Energy Transfer (homo-FRET). The homo-FRET efficiency observed from fibrillar structures in mouse embryonal fibroblasts agrees well with that of single fibrillar F-Actin synthesized from non-muscle Actin. Higher values are observed from other structures inside the cells, representing a more dense aggregation of the F-Actin in those regions.

Keywords: F-Actin; homo-FRET; fluorescence polarization; cytoskeleton

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