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Berlin 2024 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 3: Crystallization, Nucleation and Self-Assembly I

CPP 3.9: Vortrag

Montag, 18. März 2024, 12:00–12:15, H 0110

Electric-field effects on protein phase behaviorDebes Ray1, Mahnoush Madani2, Jan K. G. Dhont1, •Florian Platten1,2, and Kyongok Kang11FZ Jülich, IBI-4, Jülich, Germany — 2HHU Düsseldorf, Faculty of Mathematics and Natural Sciences, Düsseldorf, Germany

We experimentally study the state diagram of protein solutions in the presence of a weak external alternating-current electric field. The collective behavior of the protein solutions and their response to the electric field are followed in-situ by polarized optical microscopy. The crystallization boundary and the metastable liquid-liquid coexistence curve are affected by the electric field, such that the crystallization gap is widened. Both nucleation and growth of protein crystals can be modulated by the frequency and strength of the field.

Keywords: protein phase behavior; protein crystallization; LLPS

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