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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 3: Crystallization, Nucleation and Self-Assembly I
CPP 3.9: Talk
Monday, March 18, 2024, 12:00–12:15, H 0110
Electric-field effects on protein phase behavior — Debes Ray1, Mahnoush Madani2, Jan K. G. Dhont1, •Florian Platten1,2, and Kyongok Kang1 — 1FZ Jülich, IBI-4, Jülich, Germany — 2HHU Düsseldorf, Faculty of Mathematics and Natural Sciences, Düsseldorf, Germany
We experimentally study the state diagram of protein solutions in the presence of a weak external alternating-current electric field. The collective behavior of the protein solutions and their response to the electric field are followed in-situ by polarized optical microscopy. The crystallization boundary and the metastable liquid-liquid coexistence curve are affected by the electric field, such that the crystallization gap is widened. Both nucleation and growth of protein crystals can be modulated by the frequency and strength of the field.
Keywords: protein phase behavior; protein crystallization; LLPS