Bonn 2025 – wissenschaftliches Programm
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MO: Fachverband Molekülphysik
MO 23: Cluster and Nanoparticles I (joint session MO/A)
MO 23.3: Vortrag
Donnerstag, 13. März 2025, 11:45–12:00, HS XV
Novel sample delivery system for small nanoparticles and biomolecules — •Stefanie Lenzen1,4, Lukas V. Haas1,2, Kevin Janson1, Madeline Memovich1, Jingxuan He1,2, Surya Kiran Peravali1,5,6, Lena Worbs1,2, Jannik Lübke1,2,3, Armando Estillore1, Amit K. Samanta1, and Jochen Küpper1,2,3,4 — 1Center for Free-Electron Laser Science CFEL, Deutsches Elektronen- Synchrotron DESY, Hamburg, Germany — 2Department of Physics, Universität Hamburg, Hamburg, Germany — 3Center for Ultrafast Imaging, Universität Hamburg, Hamburg, Germany — 4Department of Chemistry, Universität Hamburg, Hamburg, Germany — 5Professur für Strömungsmechanik, Helmut-Schmidt-Universität/Universität der Bundeswehr, 22043, Hamburg, Germany — 6Fakultät für Maschinenbau, Helmut-Schmidt-Universität, Hamburg, Germany
Coherent diffractive imaging with free-electron lasers promises to allow the reconstruction of the three-dimensional molecular structures of isolated particles at atomic resolution [1]. However, because of the typically low signal-to-noise ratio, this requires the collection of a large amount of diffraction patterns. Since every intercepted particle is destroyed by the intense x-ray pulse, a new and preferably identical sample particle has to be delivered into every pulse.
We present a novel injection scheme, combining electrospray ionization for aerosolization of the sample, followed by shock-freezing and focusing techniques to produce a collimated or focused nanoparticle beams of a broad variety of biological nanoparticles, ranging from large nanoparticles to small single-domain proteins. These nanoparticle beams can be further manipulated to separate, for instance, charge states or conformational states, to allow pure samples to be delivered into the x-ray focus.
[1] M. M. Seibert, et al, Nature 470, 78 (2011).
Keywords: atom physics; molecular physics; biomolecules; optical scattering microscopy; single particle imaging